Education

PhD, University of Pennsylvania (2016)
MS, National Taiwan University (2008)
BS, National Chiao Tung University (2006)   

Professional Experience

Research Interest

Biochemistry, Biophysics and Physical Chemistry.

Using total internal reflection fluorescence (TIRF) microscopy as the main tool to study the membrane proteins (1) in vitro reconstituted on the supported lipid bilayer (2) directly on the live-cell membrane.
Goal:

1. Understand signal transduction among the membrane proteins and the downstream proteins at the molecular level.
2. Interrogate the biased signaling from the agonists in GPCR signaling.
3. Study cell-cell recognition in immunology using the interface between the live-cell membrane and the support lipid bilayer. 
4. Develop novel methods to extract intact membrane proteins from the live cell and in situ reconstitute them for further studies.
5. Apply the two-dimension protein phase transition to detect the transient dimeric interaction.

Publications

1. Lin, C.-W.; Nocka, L. M.; Stinger, B. L.; DeGrandchamp, J. B.; Lew, L.J. N.; Alvarez, S; Phan, H. T.; Kondo, Y.; Kuriyan, J*; Groves J. T.*, A Two-component Protein Condensate of the EGFR Cytoplasmic Tail and Grb2 Regulates Ras Activation by SOS at the Membrane. Proc. Natl. Acad. Sci U.S.A. 2022, 119(19), e2122531119.
2. Chen, Z.†; Cao, Y.†; Lin, C.-W.; Alvarez, S.; Oh D.; Yang, P.*; Groves, J. T.*, Nanopore-Mediated Protein Delivery Enabling Three-Color Single-Molecule Tracking in Living Cells. Proc. Natl. Acad. Sci. U.S.A. 2021, 118 (5), e2012229118.
3. Lin, C.-W.; Mensa, B.; Barniol-Xicota, M.; DeGrado, W. F.; Gai, F., Activation pH and Gating Dynamics of Influenza A M2 Proton Channel Revealed by Single-Molecule Spectroscopy. Angew. Chem. Int. Ed. 2017, 56, 5283.
4. Hilaire, M. R.†; Ahmed, I. A.†; Lin, C.-W.; Gai, F., Blue Fluorescent Amino Acid for Biological Spectroscopy and Microscopy. Proc. Natl. Acad. Sci. U.S.A. 2017, 114 (23), 6005.
5. Lin, C.-W.; Gai, F. Microscopic Nucleation and Propagation Rates of an Alanine-Based α-helix.  PCCP. 2017, 19, 5028-5036.
6. Lin, C.-W.; Culik, R. M.; Gai, F., Using VIPT-Jump to Distinguish Between Different Folding Mechanisms: Application to BBL and a Trpzip. J. Am. Chem. Soc. 2013, 135 (20), 7668-7673. 
7. Lin, C.-W.; Chou, P.-T.*; Liao, Y.-H.; Lin, Y.-C.*; Chen, C.-T.; Chen, Y.-C.; Lai, C.-H.; Chen, B.-S.; Liu, Y.-H.; Wang, C.-C.; Ho, M.-L.*, Photoisomerization of a Maleonitrile-Type Salen Schiff Base and Its Application in Fine-Tuning Infinite Coordination Polymers. Chemistry – A European Journal 2010, 16 (12), 3770-3782.
8. Chen, C.-L.; Lin, C.-W.; Hsieh, C.-C.; Lai, C.-H.; Lee, G.-H.; Wang, C.-C.; Chou, P.-T., Dual Excited-State Intramolecular Proton Transfer Reaction in 3-Hydroxy-2-(pyridin-2-yl)-4H-chromen-4-one. J. Phys. Chem. A 2008, 113 (1), 205-214.
9. Hwang, K.-C.; Chen, J.-L.; Chi, Y.*; Lin, C.-W.; Cheng, Y.-M.; Lee, G.-H.; Chou, P.-T.*; Lin, S.-Y.; Shu, C.-F.*, Luminescent Osmium(II) Complexes with Functionalized 2-Phenylpyridine Chelating Ligands: Preparation, Structural Analyses, and Photophysical Properties. Inorg. Chem. 2008, 47 (8), 3307-3317.
10. Chen, J.-L.; Chang, S.-Y.; Chi, Y.*; Chen, K.; Cheng, Y.-M.; Lin, C.-W.; Lee, G.-H.; Chou, P.-T.*; Wu, C.-H.; Shih, P.-I.; Shu, C.-F.*, Pt^II Complexes with 6-(5-Trifluoromethyl-Pyrazol-3-yl)-2,2′-Bipyridine Terdentate Chelating Ligands: Synthesis, Characterization, and Luminescent Properties. Chemistry – An Asian Journal 2008, 3 (12), 2112-2123.
11. Wang, C.-C.*; Lin, W.-Z.; Huang, W.-T.; Ko, M.-J.; Lee, G.-H.; Ho, M.-L.; Lin, C.-W.; Shih, C.-W.; Chou, P.-T.*, New Supramolecular Isomers with 2D 4⁴ Square-grid and 3D 6⁵·8 Frameworks in a One-pot Synthesis; Reversible Solvent Uptake and Intriguing Luminescence Properties. Chem. Commun. 2008, (11), 1299-1301.
12. Song, Y.-H.; Chiu, Y.-C.; Chi, Y.*; Chou, P.-T.*; Cheng, Y.-M.; Lin, C.-W.; Lee, G.-H.; Carty, A. J., Synthesis, Characterization, and Photophysical Properties of Luminescent Gallium and Indium Complexes Constructed using Tridentate 6-Azolyl-2,2′-bipyridine Chelates. Organometallics 2007, 27 (1), 80-87.