Education

PhD, Max-Planck Institute,Dortmund (2007)
BS, National University of Singapore (2001)
   

Professional Experience

Research Interest

Organic Chemistry, Analytical Chemistry, Chemical Biology

Publications( Full publications )

1. Pin-Wen Fang, Yu-Chun Lin, Syuan-Yun Fan, Avijit Panja, Shun-Qiang Xu, Szu-Hsien Lee, and Kui-Thong Tan*, Protein-Labeling Fluorescent Probe for Folate Receptor α, Anal. Chem. 2023, 95, 11535–11541.
2. Shun-Qiang Xu, Zong-Yan Sie, Jung-I Hsu, and Kui-Thong Tan*, Small Plasma Membrane-Targeted Fluorescent Dye for Long-Time Imaging and Protein Degradation Analyses, Anal. Chem. 2023, 95, 15549–15555.
3. Min-Chi Chung, Yun-Qiao Liu, Bo-Lin Jian, Shun-Qiang Xu, Jhih-Jie Syu, Chin-Fa Lee, and Kui-Thong Tan*, Affinity-Switchable Interaction of Biotin and Streptavidin for the Signal-ON Detection of Small Molecules, ACS Sens. 2023, 8, 4226–4232.
4. Chien-Chi Wu, Shao-Jie Huang, Tsung-Yu Fu, Fang-Ling Lin, Xin-You Wang, Kui-Thong Tan*，Small-Molecule Modulated Affinity-Tunable Semisynthetic Protein Switches，ACS Sens. 2022, 7, 2691–2700.
5. Fang-Ling Lin, Jui-Ting Yen, Pin-Wen Fang, Shun-Qiang Xu, Jing-Cyun Lin, Kui-Thong Tan*, Protein-Labeling Fluorescent Probe Reveals Ectodomain Shedding of Transmembrane Carbonic Anhydrases, ACS Chem. Biol. 2022, 17, 3218–3228.
6. Hsiao-Jung Huang, Yu-Ting Lin, Min-Chi Chung, Yu-Hsuan Chen, and Kui-Thong Tan* Glucose and Ethanol Detection with an Affinity-Switchable Lateral Flow Assay, Anal. Chem. 2022, 94, 5084-5090.
7. Kuan‐Yu Lin, Chak Hin Lam, Xin‐Hui Lin, Jung‐I Hsu, Syuan‐Yun Fan, Nitesh K Gupta, Yu‐Chun Lin, Boon Khoon Tee, Jui‐Ping Li, Jen‐Kun Chen, Kui‐Thong Tan*, Improved Stabilities of Labeling Probes for the Selective Modification of Endogenous Proteins in Living Cells and In Vivo, Chem. Asian J. 2021, 16, 937-948.
8. Yu-Hsuan Chen, Nitesh K. Gupta, Hsiao-Jung Huang, Chak Hin Lam, Ching-Lan Huang, Kui-Thong Tan*, Affinity-Switchable Lateral Flow Assay, Anal. Chem. 2021, 93, 5556–5561.
9. Chia‐Lin Wu, Chen‐Yo Fan, Chun‐Cheng Lin, Kui‐Thong Tan*, Affinity‐switchable biotin probes for the analysis of enzymes and small reactive molecules on microarray platform, J. Chin. Chem. Soc. 2021, 68, 291-297.
10. Hsiang-Yun Huang, Syuan-Yun Fan, En-Hao Chang, ChakHin Lam, Yu-Chun Lin, Xin-Hui Lin, Nitesh K Gupta, Kui-Thong Tan*, Self-ImmolativeDifluorophenyl Ester Linker for Affinity-Based Fluorescence Turn-on Protein Detection, Anal. Chem. 2020, 92, 15463–15471.
11. Hsin‐Ju Chan, Xin‐Hui Lin, Syuan‐Yun Fan, JihRu Hwu, Kui‐Thong Tan*, Rapid and Selective Labeling of Endogenous Transmembrane Proteins in Living Cells with a Difluorophenyl Ester Affinity‐Based Probe, ChemAsianJ.2020, 15,3416–3420.
12. Yu-Hsuan Chen, Wan-Ching Chien, Di-Chi Lee, Kui-Thong Tan*, Signal Amplification and Detection of Small Molecules via the Activation of Streptavidin and Biotin Recognition, Anal. Chem., 2019, 91, 12461-12467.
13. Fang‐Hong Lee, Chee Ying Chew, JihRu Hwu, Kui‐Thong Tan*, BiotinylatedFluorescent Probe for the Specific and Quantitative Determination of Blood Glucose, J. Chin. Chem. Soc. 2019, 66, 114-118.
14. Li-Yu Wei, Kai-Sheng Huang, Hsing-Hui Lin, Yung-Peng Wu, Kui-Thong Tan, Yin Yu Lee, and I-Chia Chen*,Kinetic Mechanism of Metal Enhanced Fluorescence by Gold Nanoparticle with Avidin–Biotin as Spacer and by Gold–Silver Core–Shell Nanoparticle Using Fluorescence Lifetime Image Microscopy,J. Phys. Chem. C, 2018, 122, 28431–28438.
15. Tzu‐Hsuan Chung, Yung‐Peng Wu, Chee Ying Chew, ChakHin Lam, and Kui‐Thong Tan*, Imaging and Quantification of Secreted Peroxynitrite at the Cell Surface by a Streptavidin–Biotin‐Controlled Binding Probe, Chembiochem, 2018, 19, 2584-2590.
16. Hsiang-Jung Chen,Chee Ying Chew,En-Hao Chang,Yu-Wei Tu,Li-Yu Wei,Bo-Han Wu,Chien-Hung Chen,Ya-Ting Yang,Su-Chin Huang,Jen-Kun Chen,I-Chia Chen,*and Kui-Thong Tan*,S-CisDiene Conformation: A New Bathochromic Shift Strategy forNear-Infrared Fluorescence Switchable Dye and the ImagingApplications, J. Am. Chem. Soc.2018, 140, 5224−5234.
17. Yung-Peng Wu,  Chee Ying Chew,  Tian-Neng Li,  Tzu-Hsuan Chung,  En-Hao Chang,  ChakHin Lam  and  Kui-Thong Tan*, Target-Activated Streptavidin–Biotin Controlled Binding Probe,Chem. Sci., 2018,9, 770-776.
18. Yi-Ru Hong,ChakHin Lam,and Kui-Thong Tan*,Fluorogenic Protein Labeling Probes to Study the MorphologicalInterplay between PreLamin and Mature Lamin, Bioconjugate Chem.2017, 28, 2895−2902.
19. Silvia Scarabelli, Kui Thong Tan, Rudolf Griss, Ruud Hovius, Pier Luca D’Alessandro, Thomas Vorherr, and Kai Johnsson*, Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins, ACS Sens., 2017, 2, 1191–1197.
20. 吳詠朋, 陳相榮,陳貴通*,新穎螢光探針之發展與突破, 化學, 2017, 75, 63-77.
21. Ting-Wei Wu, Fang-Hong Lee, Ruo-CingGao, Chee Ying Chew, and Kui-Thong Tan*, Fluorescent Probe Encapsulated in Avidin Protein to Eliminate Nonspecific Fluorescence and Increase Detection Sensitivity in Blood Serum, Anal. Chem.,2016, 88, 7873–7877.
22. 江柏毅,謝佩穎,莊鈺德,李秉原,許馨云,* 陳貴通*,可快速偵測碳酸酐酶及篩選磺胺類藥物之開關型螢光探針,化學,2016, 74, 3-9.
23. Yan-SyunZeng, Ruo-CingGao, Ting-Wei Wu, Chien Cho, and Kui-Thong Tan*, Fluorescent Probe Encapsulated in SNAP-Tag Protein Cavity To Eliminate Nonspecific Fluorescence and Increase Detection Sensitivity, Bioconjugate. Chem.,2016, 27, 1872–1879.
24. Wan-Yi Lai and Kui-Thong Tan*, Environment-sensitive Fluorescent Turn-on Chemical Probe for the Specific Detection of O-Methylguanine-DNA Methyltransferase (MGMT) in Living Cells, J. Chin. Chem. Soc., 2016, 63, 688-693.
25. Wan-Ting Yu, Ting-Wei Wu, Chi-Ling Huang, I-Chia Chen, and Kui-Thong Tan*, Protein Sensing in Living Cells by Molecular Rotor-Based Fluorescence Switchable Chemical Probes, Chem. Sci.2016,7, 301-307. Times cited: 6,impact factor: 9.2, Rank: 14/155)
26. Hsiu-Ping Lai, Ruo-CingGao, Chi-Ling Huang, I-Chia Chen and Kui-Thong Tan*, Fluorescence Switchable Probes based on a Molecular Rotor for Selective Detection of Proteins and Small Molecules, Chem. Commun, 2015, 51, 16197-16200. (Times cited: 1,impact factor: 6.8,Rank: 20/155)
27. Tai-Cheng Hou, Ying-Yi Wu, Po-I Chiang, and Kui-Thong Tan*, Near-Infrared Fluorogenic Probes based on Disassembly-Induced Emission Cyanine Dye, Chem. Sci.,2015, 6, 4643-4649. (Times cited: 9, impact factor: 9.2, Rank: 14/155)
28. Chia-Wen Wang, Wan-Ting Yu, Hsiu-Ping Lai, Bing-Yuan Lee, Ruo-CingGao, Kui-Thong Tan*, Steric-Dependent Label-Free and Washing-Free Enzyme Amplified Protein Detection with Dual-Functional Synthetic Probes, Anal. Chem., 2015,87, 4231-4236. (Times cited: 3,impact factor: 5.6, Rank: 4/74)
29. Tao-Kai Liu, Pei-Ying Hsieh, Yu-De Zhuang, Chi-Yang Hsia, Chi-Ling Huang, Hsiu-Ping Lai, Hung-Sheung Lin, I-Chia Chen, Hsin-Yun Hsu*, and Kui-Thong Tan*, A Rapid SNAP-Tag Fluorogenic Probe Based on an Environment-Sensitive Fluorophore for No-Wash Live Cell Imaging, ACS Chem. Biol., 2014, 9, 2359–2365.(Times cited: 13, impact factor: 5.3,Rank: 46/289)
30. Ying-Yi Wu, Wan-Ting Yu, Tai-Cheng Hou, Tao-Kai Liu, Chi-Ling Huang, I-Chia Chen and Kui-Thong Tan*, A Selective and Sensitive Fluorescent Albumin Probe for the Determination of Urinary Albumin, Chem. Commun., 2014, 50, 11507-11510. (Times cited: 16, impact factor: 6.8,Rank: 20/155)
31. Po-Ming Shih, Tao-Kai Liu and Kui-Thong Tan*, Fluorescence Amplified Detection of Proteases by the Catalytic Activation of a Semisynthetic Sensor, Chem. Commun., 2013, 49, 6212-6214.(Times cited: 12, impact factor: 6.8,Rank: 20/155)
32. Yu-De Zhuang, Po-Yi Chiang, Chia-Wen Wang and Kui-Thong Tan*, Environment-Sensitive Fluorescent Turn-On Probes Targeting Hydrophobic Ligand-Binding Domains for Selective Protein Detection,Angew. Chem. Int. Ed. 2013,52, 8124-8128. (Selected as a hot paper).(Times cited: 64, impact factor: 11.3, Rank: 13/155)